Hi guys,
We have wireless internet in the lab now! Apparently a wireless router was put into BSIF early this week, so we should have access to UCSB's Wireless Web.
Yay!
-Kristen
Thursday, May 7, 2009
Tuesday, April 28, 2009
Carb Frenzy
Hello labbies,
I'm finishing my first carb analysis pilot run this evening, and will be needing help weighing out samples, and any extra hands that are available over the next couple of weeks.
The process takes about 2 days for each set of samples, with the first day consisting of several washes with ethanol, hot water baths, some centrifuging, and then the addition of a buffer and an enzyme which digests starch to glucose over a 24 hour period. There is some down-time between ethanol washes, but I spent most of it messing around with equipment and getting ready for the next wash - this is where extra hands would be awesome.
The second day involves one last enzymatic reaction, which uses NADH as a proxy for glucose concentration, and is measurable with UV via spectrophotometer. I haven't done this part yet, and suspect that I will be getting the hang of the spec tonight (when no one will be able to hear me cuss).
I'm still working out the kinks, and may do a second pilot run starting tomorrow morning now that I am aware of a number of procedural issues (static charge of plastic containers affecting the samples, hot water bath problems, blah blah blah.)
While I'm doing all of this stuff, I will be moving around from the RiverLab, to the Schimel Lab, to the common room like a crazy person, so just give me a call if you are available and need something interesting to do (it will give you a work-out - maybe).
Gail
p.s. Aren't you proud of me Kristin? I posted my first blog.
I'm finishing my first carb analysis pilot run this evening, and will be needing help weighing out samples, and any extra hands that are available over the next couple of weeks.
The process takes about 2 days for each set of samples, with the first day consisting of several washes with ethanol, hot water baths, some centrifuging, and then the addition of a buffer and an enzyme which digests starch to glucose over a 24 hour period. There is some down-time between ethanol washes, but I spent most of it messing around with equipment and getting ready for the next wash - this is where extra hands would be awesome.
The second day involves one last enzymatic reaction, which uses NADH as a proxy for glucose concentration, and is measurable with UV via spectrophotometer. I haven't done this part yet, and suspect that I will be getting the hang of the spec tonight (when no one will be able to hear me cuss).
I'm still working out the kinks, and may do a second pilot run starting tomorrow morning now that I am aware of a number of procedural issues (static charge of plastic containers affecting the samples, hot water bath problems, blah blah blah.)
While I'm doing all of this stuff, I will be moving around from the RiverLab, to the Schimel Lab, to the common room like a crazy person, so just give me a call if you are available and need something interesting to do (it will give you a work-out - maybe).
Gail
p.s. Aren't you proud of me Kristin? I posted my first blog.
Wednesday, February 11, 2009
New email update feature
If you're interested in receiving email updates for the blog, enter your email address in the box to the right of this post. The email address you enter doesn't need to be your gmail address. You'll receive one email per day with the updates from the blog. If there are no blog updates, you won't receive an email.
-Kristen
-Kristen
Thursday, January 29, 2009
Shiny new blog!
Welcome to the RIVR lab's new blog!
We have a large lab to begin with, even if you don't take into account our many collaborations with different lab groups, schools, and universities. People are always joining and leaving, so it's difficult to keep schedules and email lists current, and on top of that someone is always going to miss a lab meeting due to other time constraints.
Enter the blog!
The idea is that if each of us takes the time to update the blog once a week or so we can remain up-to-date with what's going on instead of having a vague understanding. Instead of trying to email everyone (and inevitably leave someone out because we don't have their contact information), we can just update the blog which everyone can check. I hope that the blog may become a replacement for our group emails.
Here's what I ask:
1) After lab meeting each week the person in charge writes an entry explaining what's going on.
2) If there are any notable updates in the projects of anyone else, they can update, too. Even if it's as simple as "we're back in Nevada and will be there for the next two months!" it'd be good for everyone else to know, not just the people directly connected to that project.
3) If someone is going into the field/has a large project and needs help, they can post that request here.
Let's give this a try and see if it helps!
-Kristen
We have a large lab to begin with, even if you don't take into account our many collaborations with different lab groups, schools, and universities. People are always joining and leaving, so it's difficult to keep schedules and email lists current, and on top of that someone is always going to miss a lab meeting due to other time constraints.
Enter the blog!
The idea is that if each of us takes the time to update the blog once a week or so we can remain up-to-date with what's going on instead of having a vague understanding. Instead of trying to email everyone (and inevitably leave someone out because we don't have their contact information), we can just update the blog which everyone can check. I hope that the blog may become a replacement for our group emails.
Here's what I ask:
1) After lab meeting each week the person in charge writes an entry explaining what's going on.
2) If there are any notable updates in the projects of anyone else, they can update, too. Even if it's as simple as "we're back in Nevada and will be there for the next two months!" it'd be good for everyone else to know, not just the people directly connected to that project.
3) If someone is going into the field/has a large project and needs help, they can post that request here.
Let's give this a try and see if it helps!
-Kristen
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